Artificial climate chamber involved in the study of rice heat resistance

Identification of rice heat resistance is the basis for evaluation and utilization of rice heat-resistant germplasm resources. The scientificity of heat-resistance identification methods is the key to accurately assess and utilize heat-resistant germplasm resources. Rice is the most sensitive to high temperature throughout the growth and development of the flowering period. The high temperature test at the heading and flowering stage showed that the highest daily temperature of 35e was the critical temperature for rice heat damage. Among the yield components, the seed setting rate was the most sensitive to high temperature. In this study, 30 rice materials with different degrees of tolerance to high temperature, T226 with high temperature tolerance and T219 sensitive to high temperature were used as control materials. Artificial climate chambers were used to simulate the high temperature environment under natural conditions, and rice heat resistance identification methods were studied. Provide guidance for the scientific evaluation and utilization of rice heat-resistant germplasm resources.

When each test material was found in the ear, 2钵 of each material was transferred to the artificial climate chamber for high temperature treatment; in the artificial climate chamber, the materials were randomly arranged and re-arranged once a day to eliminate position-induced Error; the remaining 2 每 per material was still grown under natural conditions as a control. About 7 days after the material was processed, all the panicles were extracted and flowering was completed, removed from the artificial climate chamber, and returned to natural conditions.

In this study, artificial climate chambers with controllable temperature, light and humidity were used to conduct high-temperature identification tests for rice materials with different heat resistance for 2 years at the heading and flowering stage. Because this method simulates the high temperature environment under natural conditions, and can accurately control the temperature, light and humidity under high temperature conditions, the test repeatability is good and the result is accurate and reliable.

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