How to Determine the Content of Crude Fiber in Fine Powder Samples
The determination of crude fiber in fine powder samples is a difficult point in routine testing. Crude fiber measuring instrument is the most common fiber measurement method currently used. It integrates acid treatment, alkali treatment and rinsing. The operation is simpler than the national standard method GB/T 5009.10-2003, and can simultaneously measure multiple samples. The fiber tester is based on commonly used acid-base digestion samples, and then the crude fiber content of the sample is measured by the gravimetric method. Fibrometers are now widely used in the determination of crude fiber content in various foods, grains, feeds, and foods. The results of the coarse fiber tester can meet the requirements of most inspection agencies. The high-fat fine soybean sample not only contains crude fiber content, but also contains crude fat, so the fat analyzer is often used. The fat analyzer is specialized in determining the fat content, and like the crude fiber analyzer, Can measure multiple samples at the same time.
The specific process for the determination of the crude fibre content in fine powder samples: the preparation phase. Materials: Soybean samples (40 meshes, 18.18% fat); Instruments: Crude fiber analyzer, analytical balance, blast oven (40-250°C), box resistance furnace (200-1200°C), crucible Reagents: 0.128moL/L sulfuric acid solution; 0.313moL/L sodium hydroxide solution; 95% ethanol, ether, n-octanol: analytically pure; experimental phase. Sampling and degreasing: Accurately weigh 1g sample, defatted the ether three times (soak 10min; 5min; 3min), each 25mL, suction filtration. The sample was treated with acid: Add 200 mL of boiled 0.128 moL/L sulfuric acid solution and 2 drops of n-octanol, keep the micro-boiler for 30 min, and then filter the washing solution. Wash with boiling water until the washing solution is not acidic. The sample was treated with alkaline solution: add boiling boiled water of 0.313moL/L sodium hydroxide solution 200mL and 2 drops of n-octanol, keep the micro-boiling for 30min and then filter the washing solution. Wash with boiling water until the washing solution is not alkaline. Finally, the sample is dried, burned and weighed. Heat 95% ethanol 20mL a total of 3 times washing, suction filtration; ether 20mL a total of 3 times washing, filtration and evaporation of ether; move into a 105 °C blast drying oven to constant weight; then move to 550 °C Box type resistance furnace is ashed to constant weight.
The above is the determination of the crude fiber content in fine powdered soybean samples. Studies have shown that the results of the fiber content determined by this method are very close to those determined by the national standard method. Therefore, in actual operation, we usually choose the fiber tester to measure the crude fiber content of the sample because the instrument is convenient, fast, and highly safe. Many processes are automatically performed by the instrument.
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